Development and implementation of Grouping and Safe-by-Design approaches within regulatory frameworks


This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 646221 NanoReg 2

eNanoMapper database instance


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MTS CELL VIABILITY ASSAY DAPI Hoechst 33342 Impedance adherent cells ALAMAR BLUE CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Impedance flow cytometry NRU Ki-67 apoptosis cell cycle cleaved caspase-3 Spectrophotometric Method viability COMET COMET FPG RT-PCR MICRONUCLEUS ASSAY Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 CBMN assay Manual scoring ELISA LUMINEX Annexin V_PI (FACS) Griess Reagent Annexin V (Apoptosis) / PI (Necrosis) LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM Reaction Oxygen Species Detection DLS Nanoparticle tracking Analysis SEM supplier WAXS Batch Dispersion Quality VSSA TGA Filtration ICP-MS SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261


Expression of adhesion molecules radioactive tag labelling DHE DCFH2-DA MTT Assay WST-1 IL6, TNF, etc By ELISA Cytokinesis Block Micronucleus Cytome (CBMN Cyt) COMET NanoTest FeCell Chemical composition of particles flow cytometry LDH Propidium Iodide uptake fluorescent rhodamine-labeled TEM SAED DHE (Dihydroethidium) and Hoechst 33342


WST-1 LDH ALAMAR BLUE COMET Electron Paramagnetic Resonance (EPR) Fibroblast proliferation


TEER NRU Resazurin LDH CFE Embryonic Stem Cell Test WST-1 ELISA Internalization of latex beads by cells, macrophage activity, inflammation Prothrombotic effects upon systemic administration Differential Centrifugal Sedimentation (DCS) SEM X-ray Photoelectron Spectroscopy (XPS) Electron Paramagnetic Resonance (EPR) TGA ALAMAR BLUE CFDA-AM OECD TG 202 OECD TG 225 OECD TG 217 Plant root length elongation Genotoxicity MICRONUCLEUS ASSAY ICP-MS ICP-OES DLS XRD Porosity OECD TG 210 OECD TG 201 systemic administration


MICRONUCLEUS ASSAY COMET MLA-TK COMET FPG RICC TCC (Coulter counter) TRYPAN BLUE RN XTT ICP-OES TEM SAXS TGA autopsy analysis blood kinetics analysis EDS DLS BET animal info Flow cytometry with PI staining XRD


COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Merck