Advanced High Aspect Ratio and Multicomponent materials: towards comprehensive intelLigent tEsting and Safe by design Strategies
https://www.harmless-project.eu/
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 953183 HARMLESS
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NPO_1373 CHEBI_51050 NPO_1892 NPO_1542 NPO_354 ENM_9000006 CHEBI_133349 CHEBI_133326 CHEBI_59999 CHEBI_18246 NPO_606 NPO_157 NPO_943 CHEBI_3311 NPO_1550 CHEBI:18246 CHEBI_51135 CHEBI_133333 CHEBI_133337 CHEBI_133340 CHEBI_33418 NPO_401 ENM_9000007nanoreg2
CHEBI_51050 NPO_1373 NPO_1542 NPO_354 NPO_1892 CHEBI_133326 ENM_9000006 ENM_9000005 NPO_199calibrate
NPO_1373 NPO_1544 CHEBI_59999 CHEBI_82297cananolab
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CHEBI_51050 NPO_1892 ENM_9000241 NPO_606 NPO_1373 CHEBI_46666 CHEBI_82297omics
patrols
CHEBI_46727 CHEBI_133326 CHEBI_51050 NPO_1542 NPO_1892 CHEBI_59999 ENM_9000006 NPO_1544harmless
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MTS CELL VIABILITY ASSAY DAPI Hoechst 33342 Impedance adherent cells ALAMAR BLUE CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Impedance flow cytometry NRU Ki-67 apoptosis cell cycle cleaved caspase-3 Spectrophotometric Method viability COMET COMET FPG RT-PCR MICRONUCLEUS ASSAY Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 CBMN assay Manual scoring ELISA LUMINEX Annexin V_PI (FACS) Griess Reagent Annexin V (Apoptosis) / PI (Necrosis) LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM Reaction Oxygen Species Detection DLS Nanoparticle tracking Analysis SEM supplier WAXS Batch Dispersion Quality VSSA TGA Filtration ICP-MS SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261nanoreg2
COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Merckcalibrate
CellTiter-Glo (CTG) Automated hematology analyzer Short term oral toxicity Wright-Giemsa modified staining & optical microscopy Plasma (Heparin) Jejunum BAL cell celularity Histopathology COMET MICRONUCLEUS ASSAY AO Fluorescence Hyperspectral image analysis Quantitative PCR EPI-200-SIT OCL-200-EIT TEM DLS Supplier Life-history zFET Acridine Orange (AO) n.a.cananolab
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MTT Assay ALAMAR BLUE Calcein-Ethidium LDH CFE Cytokinesis Block Micronucleus Cytome (CBMN Cyt) DAN fluorimetric assay ELISA COMET CTA DCFH2-DA GSH MDA Electron Paramagnetic Resonance (EPR) Haemolysis assay TEM/SEM DLS Z-Potential in HAM'S-F12 Z-Potential in Water electroacoustic analysis on naturally concentrated samples Raman Spectroscopy UV TEER X-ray Photoelectron Spectroscopy (XPS) Infrared spectroscopy BET Z-Potential in DMEMomics
patrols
Dextran Blue IL-8 ELISA IL-6 ELISA Human IL-6 DuoSet ELISA Human IL-8 DuoSet ELISA Human TNF-alpha DuoSet ELISA BCG Albumin Assay Urea Assay Kit Micronucleus Assay Cytokinesis-Block Proliferation Index TRYPAN BLUE Human IL-8/CXCL8 DuoSet ELISA Trypan Blue assay following 3D HepG2 spheroid repeated does ENM exposure Adenylate Kinase ToxiLight Assayharmless
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CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDMnanoreg2
CACO-2 A549 CALU-3 Caki-1 Hep3B NRK-52e carp leukocyte cell linecalibrate
BEAS-2B Platelets Red blood cells Hemoglobin White blood cells Granulocytes Lymphocytes Monocytes Polymorphonuclear basophils Polymorphonuclear eosinophils Polymorphonuclear neutrophils BAL cells BAL fluid cells Liver tissue Lung tissuecananolab
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A549 BALB/3T3 RAW 264.7 cell-free sheep erythrocytes CALU-3omics
patrols
A549 H441+dTHP-1 H441 A549+dTHP-1 NCI-H441 HepG2 PHHharmless